Relationship between apoptotic markers in semen from fertile men and demographic, hormonal and seminal characteristics
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Relationship between apoptotic markers in semen from fertile men and demographic, hormonal and seminal characteristics. / Specht, Ina; Spanò, Marcello; Hougaard, Karin S; Manicardi, Gian C; Bizzaro, Davide; Toft, Gunnar; Giwercman, Aleksander; Bonde, Jens Peter Ellekilde.
I: Asian Journal of Andrology, Bind 14, Nr. 6, 2012, s. 890-6.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Relationship between apoptotic markers in semen from fertile men and demographic, hormonal and seminal characteristics
AU - Specht, Ina
AU - Spanò, Marcello
AU - Hougaard, Karin S
AU - Manicardi, Gian C
AU - Bizzaro, Davide
AU - Toft, Gunnar
AU - Giwercman, Aleksander
AU - Bonde, Jens Peter Ellekilde
PY - 2012
Y1 - 2012
N2 - Apoptosis in the testis has two putative roles during normal spermatogenesis; limitation of the germ cell population to numbers that can be supported by the Sertoli cells, and, possibly, selective depletion of meiotic and postmeiotic abnormal germ cells. We investigated the demographic and biological correlates of the pro-apoptotic marker Fas and the anti-apoptotic marker Bcl-xL in sperm cells of fertile men. Six hundred and four men from Greenland, Poland and Ukraine were consecutively enrolled during their pregnant wife's antenatal visits. Semen analysis was performed as recommended by the World Health Organization. Immunofluorescence coupled to flow cytometry was utilized for detection of apoptotic markers in the sperm cell. DNA damage was assessed by flow cytometry using both the sperm chromatin structure assay (SCSA) and the terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assay. The percentage of Fas-positive sperm cells was higher in men with high total sperm count (P
AB - Apoptosis in the testis has two putative roles during normal spermatogenesis; limitation of the germ cell population to numbers that can be supported by the Sertoli cells, and, possibly, selective depletion of meiotic and postmeiotic abnormal germ cells. We investigated the demographic and biological correlates of the pro-apoptotic marker Fas and the anti-apoptotic marker Bcl-xL in sperm cells of fertile men. Six hundred and four men from Greenland, Poland and Ukraine were consecutively enrolled during their pregnant wife's antenatal visits. Semen analysis was performed as recommended by the World Health Organization. Immunofluorescence coupled to flow cytometry was utilized for detection of apoptotic markers in the sperm cell. DNA damage was assessed by flow cytometry using both the sperm chromatin structure assay (SCSA) and the terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assay. The percentage of Fas-positive sperm cells was higher in men with high total sperm count (P
U2 - 10.1038/aja.2012.76
DO - 10.1038/aja.2012.76
M3 - Journal article
C2 - 23064689
VL - 14
SP - 890
EP - 896
JO - Asian Journal of Andrology
JF - Asian Journal of Andrology
SN - 1008-682X
IS - 6
ER -
ID: 48431994