Reference ranges of 17-hydroxyprogesterone, DHEA, DHEAS, androstenedione, total and free testosterone determined by TurboFlow-LC-MS/MS and associations to health markers in 304 men

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Reference ranges of 17-hydroxyprogesterone, DHEA, DHEAS, androstenedione, total and free testosterone determined by TurboFlow-LC-MS/MS and associations to health markers in 304 men. / Jensen, L.S.; Johannsen, T H; Holmboe, Stine Agergaard; Søeborg, T; Petersen, Jørgen Holm; Andersson, AM; Aadahl, Mette; Linneberg, Allan René; Juul, Anders.

I: Clinica Chimica Acta, Bind 454, 15.02.2016, s. 82-88.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Jensen, LS, Johannsen, TH, Holmboe, SA, Søeborg, T, Petersen, JH, Andersson, AM, Aadahl, M, Linneberg, AR & Juul, A 2016, 'Reference ranges of 17-hydroxyprogesterone, DHEA, DHEAS, androstenedione, total and free testosterone determined by TurboFlow-LC-MS/MS and associations to health markers in 304 men', Clinica Chimica Acta, bind 454, s. 82-88. https://doi.org/10.1016/j.cca.2015.12.042

APA

Jensen, L. S., Johannsen, T. H., Holmboe, S. A., Søeborg, T., Petersen, J. H., Andersson, AM., Aadahl, M., Linneberg, A. R., & Juul, A. (2016). Reference ranges of 17-hydroxyprogesterone, DHEA, DHEAS, androstenedione, total and free testosterone determined by TurboFlow-LC-MS/MS and associations to health markers in 304 men. Clinica Chimica Acta, 454, 82-88. https://doi.org/10.1016/j.cca.2015.12.042

Vancouver

Jensen LS, Johannsen TH, Holmboe SA, Søeborg T, Petersen JH, Andersson AM o.a. Reference ranges of 17-hydroxyprogesterone, DHEA, DHEAS, androstenedione, total and free testosterone determined by TurboFlow-LC-MS/MS and associations to health markers in 304 men. Clinica Chimica Acta. 2016 feb. 15;454:82-88. https://doi.org/10.1016/j.cca.2015.12.042

Author

Jensen, L.S. ; Johannsen, T H ; Holmboe, Stine Agergaard ; Søeborg, T ; Petersen, Jørgen Holm ; Andersson, AM ; Aadahl, Mette ; Linneberg, Allan René ; Juul, Anders. / Reference ranges of 17-hydroxyprogesterone, DHEA, DHEAS, androstenedione, total and free testosterone determined by TurboFlow-LC-MS/MS and associations to health markers in 304 men. I: Clinica Chimica Acta. 2016 ; Bind 454. s. 82-88.

Bibtex

@article{27a18a0a011844b2b96d21d4862709dd,

title = "Reference ranges of 17-hydroxyprogesterone, DHEA, DHEAS, androstenedione, total and free testosterone determined by TurboFlow-LC-MS/MS and associations to health markers in 304 men",

abstract = "We report reference ranges based on LC-MS/MS for testosterone (T), free testosterone (FT) and its precursors, i.e. 17-hydroxyprogesterone (17-OHP), dehydroepiandrosterone (DHEA), DHEA-sulfate (DHEAS) and androstenedione (Adione), in relation to different health markers and lifestyle factors. The study was based on 304 healthy men aged 30-61 years participating in a population-based cross-sectional study (Health2008). Examination program consisted of a clinical examination, completion of a self-administered questionnaire and blood sampling. Steroid metabolites were measured by a validated and sensitive LC-MS/MS method. Older age-groups were significantly associated with decreased concentrations of DHEA, DHEAS, Adione, and FT, while no significant associations with age were shown for 17-OHP or T. Participants with BMI≥30 kg/m(2) had lower age-related steroid metabolite z-scores compared to participants with BMI<30 kg/m(2), i.e. 17-OHP: -0.51 vs. 0.08 (p<0.001); DHEA: -0.27 vs. 0.09 (p=0.014); Adione: -0.29 vs. 0.09 (p=0.012); T: -0.99 vs. 0.14 (p<0.001); and FT -0.55 vs. 0.05 (p<0.001), respectively. In conclusion, this large study on serum steroid metabolites and concomitant assessment of health markers in healthy men provides age-related reference ranges, and furthermore evaluates the impact of lifestyle factors and metabolic syndrome on androgen metabolite levels.",

keywords = "Journal Article, Research Support, Non-U.S. Gov't",

author = "L.S. Jensen and Johannsen, {T H} and Holmboe, {Stine Agergaard} and T S{\o}eborg and Petersen, {J{\o}rgen Holm} and AM Andersson and Mette Aadahl and Linneberg, {Allan Ren{\'e}} and Anders Juul",

year = "2016",

month = feb,

day = "15",

doi = "10.1016/j.cca.2015.12.042",

language = "English",

volume = "454",

pages = "82--88",

journal = "Clinica Chimica Acta",

issn = "0009-8981",

publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Reference ranges of 17-hydroxyprogesterone, DHEA, DHEAS, androstenedione, total and free testosterone determined by TurboFlow-LC-MS/MS and associations to health markers in 304 men

AU - Jensen, L.S.

AU - Johannsen, T H

AU - Holmboe, Stine Agergaard

AU - Søeborg, T

AU - Petersen, Jørgen Holm

AU - Andersson, AM

AU - Aadahl, Mette

AU - Linneberg, Allan René

AU - Juul, Anders

PY - 2016/2/15

Y1 - 2016/2/15

N2 - We report reference ranges based on LC-MS/MS for testosterone (T), free testosterone (FT) and its precursors, i.e. 17-hydroxyprogesterone (17-OHP), dehydroepiandrosterone (DHEA), DHEA-sulfate (DHEAS) and androstenedione (Adione), in relation to different health markers and lifestyle factors. The study was based on 304 healthy men aged 30-61 years participating in a population-based cross-sectional study (Health2008). Examination program consisted of a clinical examination, completion of a self-administered questionnaire and blood sampling. Steroid metabolites were measured by a validated and sensitive LC-MS/MS method. Older age-groups were significantly associated with decreased concentrations of DHEA, DHEAS, Adione, and FT, while no significant associations with age were shown for 17-OHP or T. Participants with BMI≥30 kg/m(2) had lower age-related steroid metabolite z-scores compared to participants with BMI<30 kg/m(2), i.e. 17-OHP: -0.51 vs. 0.08 (p<0.001); DHEA: -0.27 vs. 0.09 (p=0.014); Adione: -0.29 vs. 0.09 (p=0.012); T: -0.99 vs. 0.14 (p<0.001); and FT -0.55 vs. 0.05 (p<0.001), respectively. In conclusion, this large study on serum steroid metabolites and concomitant assessment of health markers in healthy men provides age-related reference ranges, and furthermore evaluates the impact of lifestyle factors and metabolic syndrome on androgen metabolite levels.

AB - We report reference ranges based on LC-MS/MS for testosterone (T), free testosterone (FT) and its precursors, i.e. 17-hydroxyprogesterone (17-OHP), dehydroepiandrosterone (DHEA), DHEA-sulfate (DHEAS) and androstenedione (Adione), in relation to different health markers and lifestyle factors. The study was based on 304 healthy men aged 30-61 years participating in a population-based cross-sectional study (Health2008). Examination program consisted of a clinical examination, completion of a self-administered questionnaire and blood sampling. Steroid metabolites were measured by a validated and sensitive LC-MS/MS method. Older age-groups were significantly associated with decreased concentrations of DHEA, DHEAS, Adione, and FT, while no significant associations with age were shown for 17-OHP or T. Participants with BMI≥30 kg/m(2) had lower age-related steroid metabolite z-scores compared to participants with BMI<30 kg/m(2), i.e. 17-OHP: -0.51 vs. 0.08 (p<0.001); DHEA: -0.27 vs. 0.09 (p=0.014); Adione: -0.29 vs. 0.09 (p=0.012); T: -0.99 vs. 0.14 (p<0.001); and FT -0.55 vs. 0.05 (p<0.001), respectively. In conclusion, this large study on serum steroid metabolites and concomitant assessment of health markers in healthy men provides age-related reference ranges, and furthermore evaluates the impact of lifestyle factors and metabolic syndrome on androgen metabolite levels.

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.cca.2015.12.042

DO - 10.1016/j.cca.2015.12.042

M3 - Journal article

C2 - 26765096

VL - 454

SP - 82

EP - 88

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

SN - 0009-8981

ER -

ID: 164614457