Inter-laboratory validation of procedures for measuring 8-oxo-7,8-dihydrooxoguanine/8-oxo-7,8-dihydro-2`-deoxyguanosine in DNA
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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Inter-laboratory validation of procedures for measuring 8-oxo-7,8-dihydrooxoguanine/8-oxo-7,8-dihydro-2`-deoxyguanosine in DNA. / Collins, A.R.; Gedik, C.M.; Wood, S.; White, A.; Bubois, J.; Duez, P.; Rees, J.F.; Legall, R.; Degand, L.; Loft, Steffen; Jensen, A.; Poulsen, H.; Weimann, A.; Jensen, B.R.; Cadet, J.; Douki, Thierry; Ravanat, J.L.; Faure, H.; Tripier, M.; Morel, I.; Sergent, O.; Cillard, P.; Morin, B.; Epe, B.; Phoa, N.; Hartwig, A.; Pelzer, A.; Dolara, P.; Casalini, C.; Guglielmi, F.; Luceri, C.; Kasai, H.; Kido, R.; Olinski, R.; Bialkowski, K.; Duracková, Z.; Hlinchikova, L.; Korytar, P.; Dusinska, M.; Mislanova, C.; Vina, J.; Lloret, A.; Möller, L.; Hofer, T.; Gremaud, E.; Fay, L.; Stadler, R.; Eakins, J.; Progna, F.; O`Brien, J.; Elliott, R.; Astley, S.B.; Bailley, A.; Herbert, K.; Chauhan, D.; Kelly, F.; Dunster, C.; Lunec, J.; Podmore, I.; Patel, P.; Johnson, S.; Evans, M.; White, A.; Tyrrell, R.; Gordon, Matthew; Wild, Chris; Hardie, Laura.
I: Free Radical Research, Nr. vol. 36, 2002, s. 239-245.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Inter-laboratory validation of procedures for measuring 8-oxo-7,8-dihydrooxoguanine/8-oxo-7,8-dihydro-2`-deoxyguanosine in DNA
AU - Collins, A.R.
AU - Gedik, C.M.
AU - Wood, S.
AU - White, A.
AU - Bubois, J.
AU - Duez, P.
AU - Rees, J.F.
AU - Legall, R.
AU - Degand, L.
AU - Loft, Steffen
AU - Jensen, A.
AU - Poulsen, H.
AU - Weimann, A.
AU - Jensen, B.R.
AU - Cadet, J.
AU - Douki, Thierry
AU - Ravanat, J.L.
AU - Faure, H.
AU - Tripier, M.
AU - Morel, I.
AU - Sergent, O.
AU - Cillard, P.
AU - Morin, B.
AU - Epe, B.
AU - Phoa, N.
AU - Hartwig, A.
AU - Pelzer, A.
AU - Dolara, P.
AU - Casalini, C.
AU - Guglielmi, F.
AU - Luceri, C.
AU - Kasai, H.
AU - Kido, R.
AU - Olinski, R.
AU - Bialkowski, K.
AU - Duracková, Z.
AU - Hlinchikova, L.
AU - Korytar, P.
AU - Dusinska, M.
AU - Mislanova, C.
AU - Vina, J.
AU - Lloret, A.
AU - Möller, L.
AU - Hofer, T.
AU - Gremaud, E.
AU - Fay, L.
AU - Stadler, R.
AU - Eakins, J.
AU - Progna, F.
AU - O`Brien, J.
AU - Elliott, R.
AU - Astley, S.B.
AU - Bailley, A.
AU - Herbert, K.
AU - Chauhan, D.
AU - Kelly, F.
AU - Dunster, C.
AU - Lunec, J.
AU - Podmore, I.
AU - Patel, P.
AU - Johnson, S.
AU - Evans, M.
AU - White, A.
AU - Tyrrell, R.
AU - Gordon, Matthew
AU - Wild, Chris
AU - Hardie, Laura
N1 - Paper id:: 12071341
PY - 2002
Y1 - 2002
N2 - The aim of ESCODD, a European Commission funded Concerted Action, is to improve the precision and accuracy of methods for measuring 8-oxo-7,8-dihydroguanine (8-oxoGua) or the nucleoside (8-oxodG). On two occasions, participating laboratories received samples of different concentrations of 8-oxodG for analysis. About half the results returned (for 8-oxodG) were within 20% of the median values. Coefficients of variation (for three identical samples) were commonly around 10%. A sample of calf thymus DNA was sent, dry, to all laboratories. Analysis of 8-oxoGua/8-oxodG in this sample was a test of hydrolysis methods. Almost half the reported results were within 20% of the median value, and half obtained a CVof less than 10%. In order to test sensitivity, as well as precision, DNA was treated with photosensitiser and light to introduce increasing amounts of 8-oxoGua and samples were sent to members. Median values calculated from all returned results were 45.6 (untreated), 53.9, 60.4 and 65.6 8-oxoGua/10(6) Gua; only seven laboratories detected the increase over the whole range, while all but one detected a dose response over two concentration intervals. Results in this trial reflect a continuing improvement in precision and accuracy. The next challenge will be the analysis of 8-oxodG in DNA isolated from cells or tissue, where the concentration is much lower than in calf thymus DNA.
AB - The aim of ESCODD, a European Commission funded Concerted Action, is to improve the precision and accuracy of methods for measuring 8-oxo-7,8-dihydroguanine (8-oxoGua) or the nucleoside (8-oxodG). On two occasions, participating laboratories received samples of different concentrations of 8-oxodG for analysis. About half the results returned (for 8-oxodG) were within 20% of the median values. Coefficients of variation (for three identical samples) were commonly around 10%. A sample of calf thymus DNA was sent, dry, to all laboratories. Analysis of 8-oxoGua/8-oxodG in this sample was a test of hydrolysis methods. Almost half the reported results were within 20% of the median value, and half obtained a CVof less than 10%. In order to test sensitivity, as well as precision, DNA was treated with photosensitiser and light to introduce increasing amounts of 8-oxoGua and samples were sent to members. Median values calculated from all returned results were 45.6 (untreated), 53.9, 60.4 and 65.6 8-oxoGua/10(6) Gua; only seven laboratories detected the increase over the whole range, while all but one detected a dose response over two concentration intervals. Results in this trial reflect a continuing improvement in precision and accuracy. The next challenge will be the analysis of 8-oxodG in DNA isolated from cells or tissue, where the concentration is much lower than in calf thymus DNA.
U2 - 10.1080/10715760290019246
DO - 10.1080/10715760290019246
M3 - Journal article
SP - 239
EP - 245
JO - Free Radical Research
JF - Free Radical Research
SN - 1071-5762
IS - vol. 36
ER -
ID: 139535